Medium data

Search for medium no. = [1278].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1278   MIZ13 MEDIUM

Modified Wolfe's solution (see Medium No. 628)  1  L
Trace minerals (see Medium No. 151)  10.0     ml
MES  1.95    g
NaHCO3  0.83    g
Sodium acetate  1.36    g
Sodium pyruvate  1.1     g
Sodium fumarate  3.2     g
Mix components thoroughly and adjust pH 6.2. Autoclave under a N2 atmosphere. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Trace vitamins* (see Medium No. 197)  10.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml