Medium data

Search for medium no. = [1274].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


K2HPO4  2.9     g
KH2PO4  1.5     g
Urea  2.1     g
Yeast extract  6.0     g
CaCl2·2H2O  0.01    g
Na2CO3  4.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  0.5     mg
Mineral solution (see below)  0.2     ml
Distilled water  900.0     ml
Mix components and adjust pH to 7.0. Distribute the medium in culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2 and seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere):
10% (w/v) Colloidal chitin solution  100.0     ml
Mineral solution:
MgCl2·6H2O  25.0     g
CaCl2·2H2O  37.5     g
FeSO4·7H2O  0.312   g
Distilled water  1.0     L
Colloidal chitin solution can be replaced by 10% (w/v) chitin or 0.2 M cellobiose solution.