Medium data

Search for medium no. = [1264].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1264   

Yeast extract (BD-Difco)  0.05    g
Sodium formate  0.2     g
Sodium acetate  0.2     g
Bacto peptone (BD-Difco)  0.25    g
Sodium lactate solution (approx. 60%)  0.2     ml
Sodium pyruvate  0.2     g
Disodium succinate·6H2O  0.2     g
DL-Malic acid  0.2     g
Fumaric acid  0.2     g
Trisodium citrate·2H2O  0.2     g
KCl  5.4     g
K2HPO4  0.3     g
CaCl2·2H2O  0.29    g
NaNO3  0.27    g
MgCl2·6H2O  0.1     g
Na2CO3  9.0     g
Distilled water  1.0     L
After autoclaving, adjust pH to 10.0 with sterilized 10% Na2CO3 solution.



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