Medium data

Search for medium no. = [1254].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1254   HALOARCHAEAL COMPLEX MEDIUM PH 9.0

Yeast extract  0.05    g
Fish peptone (Fluka)  0.25    g
Sodium formate  0.2     g
Sodium acetate  0.2     g
Sodium lactate  0.2     g
Sodium pyruvate  0.2     g
Sodium succinate  0.2     g
Sodium malate  0.2     g
Sodium fumarate  0.2     g
Sodium citrate  0.2     g
KCl  5.4     g
K2HPO4  0.2     g
CaCl2·H2O  0.1     g
NaNO3  0.5     g
MgCl2·6H2O  0.1     g
NaCl  184.0     g
Dissolve components in distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574). After autoclaving, adjust pH to 9.0 by the addition of 10% (w/v) Na2CO3 solution (autoclaved).



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