Medium data

Search for medium no. = [1254].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1254 HALOARCHAEAL COMPLEX MEDIUM PH 9.0

Yeast extract	`0.05`	g
Fish peptone (Fluka)	`0.25`	g
Sodium formate	`0.2`	g
Sodium acetate	`0.2`	g
Sodium lactate	`0.2`	g
Sodium pyruvate	`0.2`	g
Sodium succinate	`0.2`	g
Sodium malate	`0.2`	g
Sodium fumarate	`0.2`	g
Sodium citrate	`0.2`	g
KCl	`5.4`	g
K₂HPO₄	`0.2`	g
CaCl₂·H₂O	`0.1`	g
NaNO₃	`0.5`	g
MgCl₂·6H₂O	`0.1`	g
NaCl	`184.0`	g

Dissolve components in distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574). After autoclaving, adjust pH to 9.0 by the addition of 10% (w/v) Na₂CO₃ solution (autoclaved).