Medium data

Search for medium no. = [1252].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1252 HALOARCHAEAL COMPLEX MEDIUM PH 7.0

Yeast extract	`0.05`	g
Fish peptone (Fluka)	`0.25`	g
Sodium formate	`0.2`	g
Sodium acetate	`0.2`	g
Sodium lactate	`0.2`	g
Sodium pyruvate	`0.2`	g
Sodium succinate	`0.2`	g
Sodium malate	`0.2`	g
Sodium fumarate	`0.2`	g
Sodium citrate	`0.2`	g
KCl	`5.4`	g
K₂HPO₄	`0.2`	g
CaCl₂·H₂O	`0.29`	g
NH₄Cl	`0.27`	g
MgSO₄·7H₂O	`26.8`	g
MgCl₂·6H₂O	`23.0`	g
NaCl	`184.0`	g

Dissolve components in distilled water and bring volume to 1.0 L. Adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574).