Medium data

Search for medium no. = [1249].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1249   TYG-ACETATE MEDIUM

Tryptone  10.0      g
Beef extract  10.0      g
Yeast extract  5.0      g
Malt extract  150.0      g
Glucose  20.0      g
Diammonium hydrogen citrate  2.0      g
Sodium acetate  5.0      g
K2HPO4  2.0      g
MnSO4·xH2O  0.19     g
MgSO4·7H2O  0.58     g
Tween 80  1.0      ml
L-Cysteine·HCl·H2O  1.0      g
Distilled water  1.0      L
After autoclaving, aseptically add 20 ml acetic acid (filter-sterilized).