Medium data

Search for medium no. = [1247].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1247   R2A MEDIUM WITH 25% SALT

Yeast extract (BD-Difco)  0.5     g
Proteose peptone No. 3 (BD-Difco)  0.5     g
Casamino acids (BD-Difco)  0.5     g
Glucose  0.5     g
Soluble starch  0.5     g
Sodium pyruvate  0.3     g
K2HPO4  0.3     g
MgSO4·7H2O  0.05    g
30% Seawater solution (see below)  833.0     ml
Distilled water  167.0     ml
Adjust pH to 7.5.
30% Seawater solution:
NaCl  234.0     g
MgCl2·6H2O  39.0     g
MgSO4·7H2O  61.0     g
KCl  6.0     g
CaCl2·2H2O  1.3     g
NaBr  0.7     g
NaHCO3  0.2     g
Add components to distilled water and bring volume to 1.0 L.