Medium data
Search for medium no. = [1247].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1247 R2A MEDIUM WITH 25% SALT
| Yeast extract (BD-Difco) | 0.5 | g |
| Proteose peptone No. 3 (BD-Difco) | 0.5 | g |
| Casamino acids (BD-Difco) | 0.5 | g |
| Glucose | 0.5 | g |
| Soluble starch | 0.5 | g |
| Sodium pyruvate | 0.3 | g |
| K2HPO4 | 0.3 | g |
| MgSO4·7H2O | 0.05 | g |
| 30% Seawater solution (see below) | 833.0 | ml |
| Distilled water | 167.0 | ml |
Adjust pH to 7.5.
30% Seawater solution:
| NaCl | 234.0 | g |
| MgCl2·6H2O | 39.0 | g |
| MgSO4·7H2O | 61.0 | g |
| KCl | 6.0 | g |
| CaCl2·2H2O | 1.3 | g |
| NaBr | 0.7 | g |
| NaHCO3 | 0.2 | g |
Add components to distilled water and bring volume to 1.0 L.
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