Medium data

Search for medium no. = [1239].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


KH2PO4  3.0     g
MgSO4·7H2O  1.0     g
CaCl2·2H2O  0.25    g
(NH4)2SO4  0.2     g
Yeast extract  0.1     g
Sucrose  5.0     g
Sulfur (powder)  4.0     g
Distilled water  1.0     L
Mix components except sulfur, yeast extract and sucrose, adjust pH to 2.5 with 10 N H2SO4 and autoclave. Separately autoclave yeast extract and sucrose and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) containing appropriate amount of sulfur, replace gas phase with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.

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