Medium data
Search for medium no. = [1239].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1239 THERMOPLASMA KAMCHATKENSIS MEDIUM
| KH2PO4 | 3.0 | g |
| MgSO4·7H2O | 1.0 | g |
| CaCl2·2H2O | 0.25 | g |
| (NH4)2SO4 | 0.2 | g |
| Yeast extract | 0.1 | g |
| Sucrose | 5.0 | g |
| Sulfur (powder) | 4.0 | g |
| Distilled water | 1.0 | L |
Mix components except sulfur, yeast extract and sucrose, adjust pH to 2.5 with 10 N H2SO4 and autoclave. Separately autoclave yeast extract and sucrose and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) containing appropriate amount of sulfur, replace gas phase with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.
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