Medium data

Search for medium no. = [123].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

123   BRIGGS LIVER BROTH (pH 5.0)

Tomato juice solution (see below)  400.0     ml
Neopeptone (BD-Difco)  15.0     g
Yeast extract (BD-Difco)  6.0     g
Liver extract (see Medium No. 13)  75.0     ml
Glucose  20.0     g
Soluble starch  0.5     g
NaCl  5.0     g
Tween 80  1.0     g
L-Cysteine·HCl·H2O  0.2     g
Distilled water  525.0     ml
Adjust pH to 5.0.
Dilute Tomato juice (Del Monte) with an equal volume of distilled water, adjust pH to 7.0 with 10% NaOH and filter with filter paper.



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