Medium data
Search for medium no. = [1217].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1217 MODIFIED REINFORCED CLOSTRIDIAL MEDIUM
| Yeast extract | 3.0 | g |
| Tryptone | 10.0 | g |
| Beef extract | 10.0 | g |
| Glucose | 5.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| NaCl | 3.0 | g |
| Sodium acetate | 3.0 | g |
| Soluble starch | 1.0 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 6.0-6.5. For preparation of solid medium, add per liter 20.0 g agar. Bring to a boil (and dissolve agar), cool down and distribute the medium into culture vessels (e.g., 10 ml medium in Balch type tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave.
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