Medium data

Search for medium no. = [1217].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Yeast extract  3.0     g
Tryptone  10.0     g
Beef extract  10.0     g
Glucose  5.0     g
L-Cysteine·HCl·H2O  0.5     g
NaCl  3.0     g
Sodium acetate  3.0     g
Soluble starch  1.0     g
Distilled water  1.0     L
Mix components thoroughly and adjust pH to 6.0-6.5. For preparation of solid medium, add per liter 20.0 g agar. Bring to a boil (and dissolve agar), cool down and distribute the medium into culture vessels (e.g., 10 ml medium in Balch type tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave.

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