Medium data
Search for medium no. = [1208].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1208 MARINE TRYPTONE YEAST EXTRACT MEDIUM
| Tryptone | 2.0 | g |
| Yeast extract | 1.0 | g |
| CaCl2·2H2O | 1.0 | g |
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.6 | g |
| MgSO4·7H2O | 4.3 | g |
| KCl | 0.5 | g |
| Sodium thioglycolate | 0.1 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.0. Bring to a boil and cool down under a N2 gas atmosphere. Distribute the medium in culture vessels (e.g., 10 ml in Balch tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
| 0.2 M Cellobiose solution | 30.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Comment: For strains JCM 32481, JCM 32483, JCM 32614 and JCM 32615, add separately autoclaved 1.0 M sodium pyruvate solution to the medium (20.0 ml per liter) instead of 0.2 M cellobiose solution.
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