Medium data
Search for medium no. = [1205].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1205 FUNDIDESULFOVIBRIO MAGNETOTACTCUS MEDIUM
| NH4Cl | 0.3 | g |
| MgSO4·7H2O | 0.1 | g |
| Modified Wolfe's mineral solution (see Medium No. 915) | 5.0 | ml |
| Casamino acids (BD-Difco) | 0.1 | g |
| Yeast extract | 0.02 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 957.0 | ml |
Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 gas atmosphere. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 0.25 M Potassium phosphate buffer (pH 7.0) | 5.6 | ml |
| Vitamin solution* (see Medium No. 915) | 5.0 | ml |
| 5% L-Cysteine·HCl·H2O solution (pH 7.0) | 8.0 | ml |
| 10 mM Fe quinate solution* (see below) | 5.0 | ml |
Distribute the medium into culture vessels (e.g., 20 ml in Balch tubes) and seal with butyl rubber stoppers.
10mM Fe quinate solution:
| FeCl3·6H2O | 0.225 | g |
| Quinic acid | 0.095 | g |
| Distilled water | 50.0 | ml |
Comment:
JCM 32405 may grow better in a modified medium by the addition of 20 mM sodium lactate and 50 μM Fe(III)•EDTA instead of casamino acids and ferric quinate. However, formation of magnetosomes in cells may be inhibited in the modified medium.
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