Medium data

Search for medium no. = [1203].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1203 TREPONEMA SACCHAROPHILUM MEDIUM

CaCl₂·2H₂O	`0.12`	g
MgSO₄·7H₂O	`0.19`	g
KH₂PO₄	`0.45`	g
K₂HPO₄	`0.45`	g
NaCl	`0.9`	g
(NH₄)₂SO₄	`0.9`	g
Yeast extract (BD-Difco)	`2.0`	g
Trypticase peptone	`2.0`	g
n-Butyric acid	`0.4`	ml
iso-Butyric acid	`0.4`	ml
DL-2-Methyl butyric acid	`0.2`	ml
n-Valeric acid	`0.2`	ml
iso-Valeric acid	`0.2`	ml
0.05% Resazurin solution	`1.0`	ml
L-Cysteine·HCl·H₂O	`1.0`	g
Na₂CO₃	`2.5`	g
Glucose	`2.0`	g
Distilled water	`1.0`	L

Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO₂ gas for 30-45 min to make it anoxic. Add cysteine and carbonate, then equilibrate the medium with 100% CO₂ gas to pH 7.0. Distribute medium under 100% CO₂ gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N₂ gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary.