Medium data

Search for medium no. = [1198].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1198   SALTWATER MEDIUM FOR OCEANIC AOA

NaCl  26.0     g
MgSO4·7H2O  5.0     g
MgCl2·6H2O  5.0     g
CaCl2·2H2O  1.5     g
KBr  0.1     g
Distilled water  980.0     ml
Mix components thoroughly and autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order:
HEPES solution (see Medium No. 1004)  10.0     ml
3 mM KH2PO4 solution  5.0     ml
1 M NaHCO3 solution*  2.0     ml
Modified trace element mixture (see Medium No. 1004)  1.0     ml
7.5 mM FeNaEDTA solution* (pH 7.0)  1.0     ml
1 M NH4Cl solution  1.0     ml
Check pH of the medium to be 7.2. Prior to use, add per liter 1.0 ml catalase solution (5-10 units/ml, filter-sterilized, freshly prepared) to the medium.
Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking.



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