Medium data
Search for medium no. = [1196].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1196 ACIDIHALOBACTER MEDIUM
Solution A:
| NaCl | 15.0 | g |
| (NH4)2SO4 | 0.2 | g |
| MgSO4·7H2O | 0.4 | g |
| K2HPO4 | 0.1 | g |
| Distilled water | 900.0 | ml |
Adjust pH 1.7 with H2SO4 and autoclave.
Solution B:
| FeSO4·7H2O | 13.9 | g |
| Distilled water | 100.0 | ml |
Adjust pH to 1.25 and autoclave at 115C for 10 min or filter-sterilize.
Solution C:
| K2S4O6 | 0.3 | g |
| Distilled water | 10.0 | ml |
Sterilize by filtration.
Solution D:
| Yeast extract | 0.1 | g |
| Distilled water | 10.0 | ml |
Sterilize by autoclaving.
Aseptically combine solutions A and B after cooling. Then, add solutions C and D to the medium.
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