Medium data

Search for medium no. = [1130].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1130   YCFA MEDIUM

Tryptone (BD-Difco)  10.0     g
Yeast extract (BD-Difco)  2.5     g
NaHCO3  4.0     g
Glucose  2.0     g
Maltose  2.0     g
Cellobiose  2.0     g
L-Cysteine·HCl·H2O  1.0     g
Mineral solution I (see below)  150.0     ml
Mineral solution II (see below)  150.0     ml
Resazurin  1.0     mg
VFA mix (see below)  6.2     ml
Hemin solution (see below)  10.0     ml
Vitamin solution I (see below)  1.0     ml
Distilled water  660.0     ml
Adjust pH to 7.45.
After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below).
Mineral solution I:
K2HPO4  3.0     g
Distilled water  1.0     L
Mineral solution II:
KH2PO4  3.0     g
(NH4)2SO4  6.0     g
NaCl  6.0     g
MgSO4·7H2O  0.6     g
CaCl2·2H2O  0.6     g
Distilled water  1.0     L
VFA mix:
Acetic acid  17.0     ml
Propionic acid  6.0     ml
n-Valeric acid  1.0     ml
iso-Valeric acid  1.0     ml
iso-Butyric acid  1.0     ml
Hemin solution:
KOH  0.28    g
Ethanol  25.0     ml
Hemin  0.1     g
Adjust volume to 100 ml with distilled water.
Vitamin solution I:
Biotin  5.0     mg
Vitamin B12  5.0     mg
p-Aminobenzoic acid  15.0     mg
Folic acid  25.0     mg
Pyridoxine·HCl  75.0     mg
Distilled water  500.0     ml
Vitamin solution II:
Thiamine·HCl  25.0     mg
Riboflavin  25.0     mg
Distilled water  500.0     ml