Medium data

Search for medium no. = [1111].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1111   ANAEROBIC ALKALINE LB MEDIUM

LB broth powder  25.0     g
Tris base  3.0     g
Distilled water  800.0     ml
Mix components thoroughly, adjust pH to 9.5 with NaOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions (autoclaved and stored under N2):
8% Na2CO3 solution  200.0     ml
5% Na2S·9H2O solution  8.0     ml