Medium data

Search for medium no. = [1107].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1107   LIQUID BASAL MEDIUM FOR METHANOSARCINA

KH2PO4  0.5     g
NH4Cl  0.4     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.05    g
NaCl  0.4     g
MOPS  4.2     g
Trace element solution (see below)  1.0     ml
Resazurin  1.0     mg
Distilled water  930.0     ml
Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  50.0     ml
Vitamin solution* (see Medium No. 944)  10.0     ml
50% (v/v) Methanol solution  10.0     ml
Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml
Readjust pH to 7.2, if necessary.
Trace elements solution:
Nitrilotriacetic acid trisodium salt monohydrate  11.1     g
FeSO4·7H2O  5.55    g
Na2SeO3·5H2O  0.3     g
CoCl2·6H2O  1.0     g
MnSO4·5H2O  1.0     g
Na2MoO4·2H2O  1.0     g
Na2WO4·2H2O  1.0     g
ZnSO4·7H2O  1.0     g
NiCl2·6H2O  3.0     g
H3BO3  0.1     g
CuSO4·5H2O  0.1     g
Distilled water  1.0     L