Medium data
Search for medium no. = [1107].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1107 LIQUID BASAL MEDIUM FOR METHANOSARCINA
| KH2PO4 | 0.5 | g |
| NH4Cl | 0.4 | g |
| MgCl2·6H2O | 0.4 | g |
| CaCl2·2H2O | 0.05 | g |
| NaCl | 0.4 | g |
| MOPS | 4.2 | g |
| Trace element solution (see below) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 930.0 | ml |
Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 50.0 | ml |
| Vitamin solution* (see Medium No. 944) | 10.0 | ml |
| 50% (v/v) Methanol solution | 10.0 | ml |
Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Readjust pH to 7.2, if necessary.
Trace elements solution:
| Nitrilotriacetic acid trisodium salt monohydrate | 11.1 | g |
| FeSO4·7H2O | 5.55 | g |
| Na2SeO3·5H2O | 0.3 | g |
| CoCl2·6H2O | 1.0 | g |
| MnSO4·5H2O | 1.0 | g |
| Na2MoO4·2H2O | 1.0 | g |
| Na2WO4·2H2O | 1.0 | g |
| ZnSO4·7H2O | 1.0 | g |
| NiCl2·6H2O | 3.0 | g |
| H3BO3 | 0.1 | g |
| CuSO4·5H2O | 0.1 | g |
| Distilled water | 1.0 | L |
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