Medium data

Search for medium no. = [1099].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1099   HEART INFUSION AGAR WITH 5% HORSE BLOOD

Heart infusion broth (BD-Difco)  25.0     g
Horse blood  50.0     ml
Agar  15.0     g
Distilled water  950.0     ml
Mix ingredients except horse blood, autoclave and cool to about 50C. Aseptically add 5% (final) sterile defibrinated horse blood. Mix and quickly dispense into sterile petri dishes.