Medium data
Search for medium no. = [1088].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1088 0.3 x HYPHOMICROBIUM MEDIUM
| K2HPO4 | 0.85 | g |
| NaH2PO4 | 0.75 | g |
| (NH4)2SO4 | 0.2 | g |
| MgSO4·7H2O | 0.07 | g |
| Visniac trace elements (see Medium No. 884) | 1.0 | ml |
| Trace vitamins (see below) | 2.0 | ml |
| Methanol | 2.0 | ml |
| Agar | 15.0 | g |
| Distilled water | 1.0 | L |
After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.
Trace vitamins:
| Vitamin B12 | 125.0 | mg |
| Thiamine·HCl | 25.0 | mg |
| Folic acid | 12.5 | mg |
| Ascorbic acid | 40.0 | mg |
| Riboflavin | 25.0 | mg |
| Niacin | 25.0 | mg |
| Pantothenic acid | 25.0 | mg |
| Distilled water | 500.0 | ml |
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved