Medium data

Search for medium no. = [1088].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1088   0.3 x HYPHOMICROBIUM MEDIUM

K2HPO4  0.85    g
NaH2PO4  0.75    g
(NH4)2SO4  0.2     g
MgSO4·7H2O  0.07    g
Visniac trace elements (see Medium No. 884)  1.0     ml
Trace vitamins (see below)  2.0     ml
Methanol  2.0     ml
Agar  15.0     g
Distilled water  1.0     L
After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.
Trace vitamins:
Vitamin B12  125.0     mg
Thiamine·HCl  25.0     mg
Folic acid  12.5     mg
Ascorbic acid  40.0     mg
Riboflavin  25.0     mg
Niacin  25.0     mg
Pantothenic acid  25.0     mg
Distilled water  500.0     ml