Medium data

Search for medium no. = [1086].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1086   PEAT MEDIUM WITH SUCROSE

Peptone (BD-Difco)  0.25    g
Yeast extract  1.0     g
Sucrose  2.0     g
Major metals (see Medium No. 923)  10.0     ml
Trace metal 1 solution (see Medium No. 923)  0.1     ml
Distilled water  1.0     L
Mix components thoroughly. For preparation of solid medium, add 15.0 g/L agar. After autoclaving and cooling (for solid medium, cooling to 55C), add the following solution (filter-sterilized):
0.5 M MES solution (pH 5.7)  40.0     ml
Aseptically distribute the medium into culture vessels and replace the gas phase with a N2-CO2 (4:1, v/v) gas atmosphere.



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