Medium data
Search for medium no. = [1063].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1063 SEA SALTS YEAST EXTRACT GLUCOSE MEDIUM
| Sea salts (Sigma) | 30.0 | g |
| KH2PO4 | 0.15 | g |
| K2HPO4 | 0.15 | g |
| NH4Cl | 2.0 | g |
| Yeast extract | 2.0 | g |
| Glucose | 5.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| NaHCO3 | 1.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components except NaHCO3 and adjust pH to 7.0. Bring to a boil, cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream and add NaHCO3. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave.
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