Medium data

Search for medium no. = [1063].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1063   SEA SALTS YEAST EXTRACT GLUCOSE MEDIUM

Sea salts (Sigma)  30.0     g
KH2PO4  0.15    g
K2HPO4  0.15    g
NH4Cl  2.0     g
Yeast extract  2.0     g
Glucose  5.0     g
L-Cysteine·HCl·H2O  0.5     g
NaHCO3  1.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components except NaHCO3 and adjust pH to 7.0. Bring to a boil, cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream and add NaHCO3. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave.