Medium data
Search for medium no. = [1055].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1055 MARINE AOB MEDIUM (A)
| NaCl | 29.22 | g |
| (NH4)2SO4 | 1.652 | g |
| MgSO4·7H2O | 7.394 | g |
| MgCl2·6H2O | 4.066 | g |
| CaCl2·2H2O | 1.470 | g |
| KCl | 0.746 | g |
| Na2CO3 | 21.2 | mg |
| NaHCO3 | 0.252 | g |
| K2HPO4 | 15.7 | mg |
| 1.0 mg/ml EDTA·Fe(III) solution | 1.0 | ml |
| Trace elements solution (see below) | 1.0 | ml |
| Phenol red (0.04%) | 2.5 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 7.0 with HCl. After autoclave, pH goes up to around 7.5.
Trace elements solution:
| Na2MoO4·2H2O | 241.95 | mg |
| MnCl2·4H2O | 197.91 | mg |
| ZnSO4·7H2O | 287.56 | mg |
| CoCl2·6H2O | 237.93 | mg |
| CuSO4·5H2O | 249.68 | mg |
| Distilled water | 1.0 | L |
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