Medium data

Search for medium no. = [1052].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1052 573C MEDIUM

Carboxymethyl cellulose, sodium salt	`2.5`	g
(NH₄)₂SO₄	`1.3`	g
KH₂PO₄	`0.37`	g
Glucose	`1.0`	g
Yeast extract (BD-Difco)	`0.25`	g
Tryptone (BD-Difco)	`0.9`	g
25% MgSO₄·7H₂O solution	`1.0`	ml
7% CaCl₂·2H₂O solution	`1.0`	ml
2% FeCl₃·6H₂O solution	`1.0`	ml
Distilled water	`1.0`	L

Adjust pH to 4.8 - 5.0. Separately autoclave MgSO₄·7H₂O solution, CaCl₂·2H₂O solution and FeCl₃·6H₂O solution. Aseptically add all solutions to the medium.