Medium data
Search for medium no. = [1047].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1047 MARINITOGA MEDIUM
| Sea salts (Sigma) | 30.0 | g |
| PIPES | 6.0 | g |
| Yeast extract | 1.0 | g |
| Tryptone (BD-Difco) | 1.0 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 7.0. Bubble the medium with N2 for 5 min, then distribute the medium into culture vessels under N2, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions aseptically and anaerobically (autoclaved and stored under a N2 gas atmosphere):
| 1.0 M Glucose solution | 14.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
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