Medium data

Search for medium no. = [1045].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1045   FASTIDIOUS ANAEROBE BROTH MEDIUM

Bacto peptone (BD-Difco)  23.0     g
NaCl  5.0     g
Soluble starch  1.0     g
NaHCO3  0.4     g
Glucose  1.0     g
Sodium pyruvate  1.0     g
L-Cysteine·HCl·H2O  0.5     g
Sodium pyrophosphate  0.25    g
L-Arginine  1.0     g
Hemin  0.01    g
Distilled water  1.0     L
Mix components, adjust pH 7.2 and autoclave. Cool medium under a N2-CO2 (4:1, v/v) gas stream and add anaerobically the following solution (filter-sterilized).
250 mM Dithiothreitol solution  4.0     ml
Aseptically and anaerobically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Readjust pH to 7.0 - 7.2.