Medium data

Search for medium no. = [1038].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1038 METHANOBACTERIUM MEDIUM (VI)

Use Medium No. 872 with final 0.4 g/L NaCl.
Comment: Sludge fluid can be replaced with a human fecal extract. The extract is prepared as follows: autoclave equal quantities of human feces and water at 121C for 30 min. Remove the sludge by centrifugation. Adjust pH of the supernatant to 7.0-7.2 and sterilize at 121C for 15 min under a N₂ atmosphere.