Medium data
Search for medium no. = [102].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
102 NUTRIENT AGAR WITH 25% SOIL EXTRACT
| Peptone | 5.0 | g |
| Beef extract | 3.0 | g |
| Soil extract (see below) | 250.0 | ml |
| Agar | 15.0 | g |
| Tap water | 750.0 | ml |
Adjust pH to 7.0.
Soil extract:
Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved