Medium data
Search for medium no. = [1011].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1011 SW-7.5 MEDIUM
| SW-25 solution (see below) | 300.0 | ml |
| Distilled water | 700.0 | ml |
| Yeast extract (BD-Difco) | 5.0 | g |
Adjust pH to 7.5 and autoclave. For preparation of solid medium, add 20.0 g/L agar.
SW-25 solution:
| NaCl | 195.0 | g |
| MgCl2·6H2O | 32.5 | g |
| MgSO4·7H2O | 51.0 | g |
| KCl | 5.0 | g |
| NaBr | 0.6 | g |
| NaHCO3 | 0.16 | g |
| CaCl2·2H2O | 0.8 | g |
Add components to distilled water and bring volume to 1.0 L.
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