Medium data

Search for medium no. = [1011].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1011   SW-7.5 MEDIUM

SW-25 solution (see below)  300.0     ml
Distilled water  700.0     ml
Yeast extract (BD-Difco)  5.0     g
Adjust pH to 7.5 and autoclave. For preparation of solid medium, add 20.0 g/L agar.
SW-25 solution:
NaCl  195.0     g
MgCl2·6H2O  32.5     g
MgSO4·7H2O  51.0     g
KCl  5.0     g
NaBr  0.6     g
NaHCO3  0.16    g
CaCl2·2H2O  0.8     g
Add components to distilled water and bring volume to 1.0 L.