Medium data

Search for medium no. = [101].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

101   BACILLUS ACIDOCALDARIUS MEDIUM

Solution A:
Yeast extract  1.0     g
(NH4)2SO4  0.2     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
KH2PO4  0.6     g
Distilled water  500.0     ml
Adjust pH to 3.0-4.0.
Solution B:
Glucose  1.0     g
Agar  20.0     g
Distilled water  500.0     ml
Autoclave Solutions A and B separately, and mix aseptically.