Medium data

Search for medium no. = [1003].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1003 ACIDIC HB-1 MEDIUM

Use Medium No. 744. Before autoclaving, adjust pH of the medium to 5.5. After autoclaving, add per liter the following solutions aseptically and anaerobically from anaerobic stocks:

10% KNO₃ solution	`20.0`	ml
20% MES (pH 5.5) solution	`25.0`	ml
3% Na₂S·9H₂O solution (neutralized)	`4.0`	ml

Otherwise, prepare the medium as described in the recipe of Medium No. 744.