Medium data

Search for medium no. = [1002].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1002   YTPS MEDIUM

Yeast extract (BD-Difco)  5.0     g
Tryptone (BD-Difco)  5.0     g
Sodium pyruvate  3.0     g
NaCl  19.0     g
MgCl2·6H2O  9.0     g
MgSO4·7H2O  0.15    g
CaCl2·2H2O  0.3     g
KCl  0.5     g
KH2PO4  0.42    g
(NH4)2SO4  0.1     g
NaBr  0.05    g
SrCl2·6H2O  0.02    g
Na2SeO4  0.02    g
Wolfe's modified mineral elixir(see Medium No. 221)  5.0     ml
Vitamin solution (see Medium No. 221)  0.5     ml
PIPES  6.05    g
L-cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Adjust pH to 7.3.
Mix ingredients, except L-cysteine·HCl·H2O, and bring to the boil while gassing with N2-H2 (95:5) gas mixture. When the color turns from blue to reddish pink, add L-cysteine·HCl·H2O during continuous gassing and boiling. Anaerobically dispense the medium in tubes under a N2-H2 (95:5) atmosphere. Stopper the tubes and autoclave.



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