JCM, IFM, GTC, and NEKKEN* established a new MALDI-TOF MS reference library ‘EMALiMB’ (Expanded MALDI-TOF MS Library for MALDI Biotyper^®) for various microbial strains. You can download the reference data file (.btmsp) via the link below and import it in the software (compatible with Bruker’s MALDI Biotyper^®). Depending on your PC environment, errors may occur while importing large .btmsp files. In such cases, please try importing the files in smaller batches by lower taxonomic groups.

EMALiMB_NBRP_Library_ver3.0 provides 2,000 data of the JCM + IFM strains (eukaryotic microbes), ca. 1,140 data of the JCM + GTC strains (prokaryotes), and 14 data of the NEKKEN strains (including Trypanosoma), etc. In this current version, the MALDI-TOF MS data of anaerobes including lactic acid bacteria and intestinal bacteria are available. Please note that the ver3.0 includes the data from the ver2.0.

This project was supported by the FY2020-2021 Fundamental Technology Upgrading Program of National BioResource Project (NBRP) of the MEXT, Japan, and we have been adding MALDI-TOF MS data in the current version of the library. The microbial strains used in this project are available from JCM, IFM, GTC, and NEKKEN.

* JCM: Japan Collection of Microorganisms, RIKEN BioResource Research Center

IFM: Medical Mycology Research Center, Chiba University

GTC: Center for Conservation of Microbial Genetic Resource, Gifu University

NEKKEN: Institute of Tropical Medicine, Nagasaki University

	EMALiMB ver2.0 (updated on Feb. 27, 2023)		EMALiMB ver3.0 (updated on Feb. 21, 2025)
Fungi	1,330 MSPs		2,000 MSPs
Ascomycota	921 MSPs		1,317 MSPs
Pezizomycotina	-		232 MSPs
Saccharomycotina	-		1,054 MSPs
Taphrinomycotina	-		31 MSPs
Basidiomycota	409 MSPs		662 MSPs
Mucoromycota	-		21 MSPs
Prototheca	14 MSPs		14 MSPs
Bacteria	390 MSPs		1,142 MSPs
Protozoa	-		14 MSPs
All	1,734 MSPs		3,170 MSPs

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