JCM, IFM, GTC, and NEKKEN* established a new MALDI-TOF MS reference library ‘EMALiMB’ (Expanded MALDI-TOF MS Library for MALDI Biotyper^®) for various microbial strains. You can download the reference data file (.btmsp) via the link below and import it in the software (compatible with Bruker’s MALDI Biotyper^®). Depending on your PC environment, errors may occur while importing large .btmsp files.

You can download the strain list here.

EMALiMB_NBRP_Library_ver3.1 provides 2,000 data of the JCM + IFM strains (eukaryotic microbes), ca. 1,140 data of the JCM + GTC strains (prokaryotes), and 14 data of the NEKKEN strains (including Trypanosoma), etc. In this current version, the MALDI-TOF MS data of anaerobes including lactic acid bacteria and intestinal bacteria are available. Please note that the ver3.1 includes the data from the ver3.0.

This project was supported by the FY2020-2021 Fundamental Technology Upgrading Program of National BioResource Project (NBRP) of the MEXT, Japan, and we have been adding MALDI-TOF MS data in the current version of the library. The microbial strains used in this project are available from JCM, IFM, GTC, and NEKKEN.

* JCM: Japan Collection of Microorganisms, RIKEN BioResource Research Center

IFM: Medical Mycology Research Center, Chiba University

GTC: Center for Conservation of Microbial Genetic Resource, Gifu University

NEKKEN: Institute of Tropical Medicine, Nagasaki University

Taxonomy	EMALiMB ver3.1 (updated on Oct. 1, 2026)
All	3,170 MSPs
Bacteria	1,142 MSPs
Bacillati	763 MSPs
Actinomycetota	202 MSPs
Actinomycetes	180 MSPs
Coriobacteriia	22 MSPs
Bacillota	561 MSPs
Bacilli	296 MSPs
Caryophanales (Bacillales)	61 MSPs
Lactobacillales	235 MSPs
Aerococcaceae	3 MSPs
Carnobacteriaceae	5 MSPs
Enterococcaceae	9 MSPs
Lactobacillaceae	184 MSPs
Streptococcaceae	34 MSPs
Clostridia	196 MSPs
Eubacteriales	98 MSPs
Lachnospirales	67 MSPs
Peptostreptococcales	31 MSPs
Erysipelotrichia	25 MSPs
Negativicutes	27 MSPs
Tissierellia	17 MSPs
Fusobacteriati	4 MSPs
Fusobacteriota	4 MSPs
Pseudomonadati	373 MSPs
Bacteroidota	183 MSPs
Bacteroidia	162 MSPs
Chitinophagia	11 MSPs
Cytophagia	1 MSPs
Flavobacteriia	1 MSPs
Sphingobacteriia	8 MSPs
Campylobacterota	21 MSPs
Lentisphaerota	2 MSPs
Myxococcota	4 MSPs
Pseudomonadota	154 MSPs
Thermodesulfobacteriota	3 MSPs
Verrucomicrobiota	6 MSPs
Thermotogati	2 MSPs
Synergistota	2 MSPs
Fungi	2,000 MSPs
Ascomycota	1,319 MSPs
Ascomycota incertae sedis	1 MSPs
Saccharomyceta	1,287 MSPs
Pezizomycotina	234 MSPs
Saccharomycotina	1,053 MSPs
Dipodascomycetes	199 MSPs
Lipomycetes	23 MSPs
Pichiomycetes	570 MSPs
Alaninales	15 MSPs
Pichiales	157 MSPs
Serinales	398 MSPs
Cephaloascaceae	1 MSPs
Debaryomycetaceae	354 MSPs
Candida/Lodderomyces clade	73 MSPs
Other clades	281 MSPs
Metschnikowiaceae	42 MSPs
Serinales incertae sedis	1 MSPs
Saccharomycetes	251 MSPs
Sporopachydermiomycetes	8 MSPs
Trigonopsidomycetes	2 MSPs
Taphrinomycotina	31 MSPs
Basidiomycota	660 MSPs
Agaricomycotina	390 MSPs
Agaricomycetes	52 MSPs
Tremellomycetes	338 MSPs
Cystofilobasidiales	41 MSPs
Filobasidiales	51 MSPs
Holtermanniales	2 MSPs
Tremellales	131 MSPs
Trichosporonales	113 MSPs
Pucciniomycotina	203 MSPs
Ustilaginomycotina	66 MSPs
Wallemiomycotina	1 MSPs
Mucoromycota	21 MSPs
Prototheca	14 MSPs
Protozoa	14 MSPs

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