Medium data
Search for medium no. = [977].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
977 VNG MEDIUM
| 10 x CMD stock solution (see below) | 100.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NH4Cl | 0.027 | g |
| HEPES | 3.57 | g |
| Distilled water | 790.0 | ml |
Mix components thoroughly, adjust pH to 8.1 with NaOH or HCl. Distribute the medium into culture vessels (e.g., 9 ml in 25 ml serum bottles/Balch tubes), seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 900 ml, autoclaved):
| 20% (w/v) Glucose | 100.0 | ml |
10 x CMD stock solution:
| Nitrilotriacetic acid | 1.0 | g |
| 5 x CMD micronutrient solution (see below) | 1.0 | ml |
| FeCl3·6H2O | 4.8 | mg |
| CaSO4·2H2O | 0.6 | g |
| MgSO4·7H2O | 1.0 | g |
| NaCl | 0.8 | g |
| KNO3 | 1.03 | g |
| NaNO3 | 6.89 | g |
| NaH2PO4 | 1.11 | g |
| Distilled water | 1.0 | L |
Do not use if exceeds 90 days shelf life.
5 x CMD micronutrient solution:
| Distilled water | 100.0 | ml |
| 6.25 N H2SO4 solution | 0.25 | ml |
| MnSO4·xH2O | 1.14 | g |
| ZnSO4·7H2O | 0.25 | g |
| H3BO3 | 0.25 | g |
| CuCl2·2H2O | 8.5 | mg |
| Na2MoO4·2H2O | 12.5 | mg |
| CoCl2·6H2O | 22.5 | mg |
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