Medium data
Search for medium no. = [968].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
968 MODIFIED CALDICELLULOSIRUPTOR MEDIUM
NH4Cl | 0.9 | g |
NaCl | 0.9 | g |
MgCl2·6H2O | 0.4 | g |
KH2PO4 | 0.75 | g |
K2HPO4 | 1.5 | g |
Trypticase peptone (BD-BBL) | 2.0 | g |
Yeast extract (BD-Difco) | 1.0 | g |
Trace element solution SL-10 (see Medium No. 433) | 1.0 | ml |
FeCl3·6H2O | 2.5 | mg |
Xylose | 1.0 | g |
L-Cysteine·HCl·H2O | 0.75 | g |
Resazurin | 0.5 | mg |
Distilled water | 1.0 | L |
Mix components, except xylose, thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under 100% N2, seal with butyl rubber stoppers and autoclave. Add xylose after autoclaving from an anoxic stock solution prepared under a N2 gas atmosphere and sterilized by filtration.
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