Medium data
Search for medium no. = [933].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
933 THERMOANAEROBACULUM AQUATICUM MEDIUM
| CaCl2·2H2O | 0.25 | g |
| KH2PO4 | 0.34 | g |
| NH4Cl | 0.34 | g |
| KCl | 0.34 | g |
| MgSO4·7H2O | 0.5 | g |
| NaCl | 0.25 | g |
| Yeast extract | 0.1 | g |
| RST trace elements (see below) | 1.0 | ml |
| 0.001% Coenzyme M solution | 0.5 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| Sodium nitrate | 0.85 | g |
| Sodium pyruvate | 1.1 | g |
| TES | 1.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave. Reduce the medium by the addition of the following solutions (autoclaved under a N2 gas atmosphere) per liter:
| 5% Na2S·9H2O solution | 8.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 8.0 | ml |
RST trace elements:
| Nitrilotriacetic acid | 2.0 | g |
| MnSO4·xH2O | 1.0 | g |
| Fe(NH4)2(SO4)2·6H2O | 0.8 | g |
| CoCl2·6H2O | 0.2 | g |
| ZnSO4·7H2O | 0.2 | g |
| CuCl2·2H2O | 0.02 | g |
| NiCl2·6H2O | 0.02 | g |
| Na2MoO4·2H2O | 0.02 | g |
| Na2SeO4 | 0.02 | g |
| Na2WO4·2H2O | 0.02 | g |
| Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid and adjust pH to 6.0 with KOH. Then proceed to add minerals.
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