Medium data
Search for medium no. = [899].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
899 THERMOBACTER ROSEUM MEDIUM
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.25 | g |
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.4 | g |
| KCl | 0.5 | g |
| CaCl2·2H2O | 0.1 | g |
| NaHCO3 | 0.1 | g |
| Resazurin | 1.0 | mg |
| Yeast extract (BD-Difco) | 2.0 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| Vitamin solution* (see below) | 1.0 | ml |
| Trace element solution* (see below) | 1.0 | ml |
| 5% Na2S·9H2O | 6.0 | ml |
Check pH to be 7.5 - 7.6.
Vitamin solution:
| p-Aminobenzoic acid | 25.0 | mg |
| Biotin | 100.0 | mg |
| Nicotinic acid | 25.0 | mg |
| DL-Calcium pantothenate | 25.0 | mg |
| Pyridoxine·HCl | 25.0 | mg |
| Folic acid | 10.0 | mg |
| Riboflavin | 25.0 | mg |
| Vitamin B12 | 0.5 | mg |
| Lipoic acid | 25.0 | mg |
| Distilled water | 1.0 | L |
Trace element solution:
| (NH4)2Fe(SO4)2·6H2O | 784.0 | mg |
| 25% HCl | 5.0 | ml |
| CoCl2·6H2O | 238.0 | mg |
| (NH4)2Ni(SO4)2•6H2O | 395.0 | mg |
| Na2MoO4·2H2O | 36.0 | mg |
| Na2WO4·2H2O | 33.0 | mg |
| ZnSO4·7H2O | 144.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| Na2SeO4 | 94.0 | mg |
| H3BO3 | 6.0 | mg |
| MnCl2·4H2O | 99.0 | mg |
| Distilled water | 995.0 | ml |
Dissolve (NH4)2Fe(SO4)2·6H2O in 25% HCl first, then dilute in distilled water. Add the remaining salts in the sequence indicated
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved