Medium data
Search for medium no. = [891].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
891 RAE MEDIUM
| Glucose | 40.0 | g |
| Peptone | 10.0 | g |
| Yeast extract | 10.0 | g |
| Citric acid | 1.5 | g |
| Na2HPO4·2H2O | 3.38 | g |
| Glacial acetic acid | 10.0 | ml |
| Ethanol | 10.0 | ml |
| Agar | 20.0 | g |
| Distilled water | 980.0 | ml |
Separately filter-sterilize glacial acetic acid and ethanol (use Teflon filters), and aseptically add to the sterile medium. Final pH should be about 3.8.
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