Medium data
Search for medium no. = [758].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
758 DETHIOSULFOVIBRIO II MEDIUM
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KH2PO4 | 1.0 | g |
| Trisodium citrate·H2O | 3.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Bacto peptone (BD-Difco) | 2.0 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix components and autoclave under a N2 atmosphere. After cooling, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
| 10% CaCl2·2H2O solution | 2.0 | ml |
| 12.5% Na2S2O3·5H2O solution* | 20.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Adjust pH of the medium to 6.7 - 6.8, if necessary.
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