Medium data
Search for medium no. = [698].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
698 ACIDIMICROBIUM MEDIUM
MgSO4·7H2O | 0.5 | g |
(NH4)2SO4 | 0.4 | g |
K2HPO4 | 0.2 | g |
KCl | 0.1 | g |
FeSO4·7H2O | 10.0 | mg |
Distilled water | 1.0 | L |
Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved):
10% Yeast extract (BD-Difco)solution | 2.5 | ml |
Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4·7H2O and adjust pH to 1.7 with H2SO4.
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