Medium data

Search for medium no. = [659].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

659   PELAGICOCCUS AGAR

Marine agar 2216 (BD-Difco)  55.1     g
R2A agar (BD-Difco)  9.1     g
Artificial seawater (see below)  750.0     ml
Distilled water  250.0     ml
Artificial seawater:
NaCl  24.0     g
MgSO4·7H2O  7.0     g
MgCl2·6H2O  5.3     g
KCl  0.7     g
CaCl2·2H2O  0.1     g
Distilled water  1.0     L
Adjust pH to 7.5.
Comment: For preparation of the liquid medium, dissolve 37.4 g/L Marine Broth 2216 (BD-Difco) and 1.6 g/L R2A broth (Daigo) in the artificial sea water.



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