Medium data

Search for medium no. = [656].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

656   TRYPTONE-YEAST EXTRACT BROTH (ISP-1) WITH 0.2% MgSO4·7H2O

Tryptone (BD-Difco)  5.0     g
Yeast extract (BD-Difco)  3.0     g
MgSO4·7H2O  2.0     g
Distilled water  1.0     L
Adjust pH to 7.0 - 7.2.
Comment: The premixed powder of Tryptone-yeast extract broth is available from Becton Dickinson & Co. as ISP Medium 1. Add MgSO4·7H2O to the medium before autoclaving.