Medium data

Search for medium no. = [607].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

607   HALOMONAS SUBGLACIESCOLA MEDIUM

NaCl  100.0     g
MgCl2·6H2O  5.0     g
MgSO4·7H2O  9.5     g
KCl  5.0     g
CaCl2·2H2O  0.2     g
(NH4)2SO4  0.1     g
KNO3  0.1     g
Peptone  5.0     g
Yeast extract  1.0     g
Distilled water  1.0     L
Adjust pH to 7.0. For preparation of solid medium, add 15.0 g/L agar. Autoclave. After cooling to 50C, add the following solutions to the medium (autoclaved or *filter-sterilized):
Metal solution (see below)  20.0     ml
Phosphate solution (see below)  20.0     ml
Trace vitamins* (see Medium No. 197)  1.0     ml
Metal solution:
Nitrilotriacetic acid  10.0     g
MgSO4·7H2O  29.7     g
CaCl2·2H2O  3.3     g
Na2MoO4·2H2O  12.7     mg
FeSO4·7H2O  99.0     mg
Metals ''44'' (see Medium No. 149)  50.0     ml
Distilled water  1.0     L
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add the minerals. Adjust final pH to 7.0.
Phosphate solution:
K2HPO4  2.5     g
KH2PO4  2.5     g
Distilled water  1.0     L



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