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591 HALF STRENGTH TRYPTICASE SOY BROTH AGAR (pH 4.0)
Solution A:
Trypticase soy broth (BBL)
15.0
g
Distilled water
500.0
ml
Adjust pH to 4.0. Solution B:
Agar
15.0
g
Distilled water
500.0
ml
After autoclaving Solutions A and B separately and cooling to 50C, aseptically mix both solutions and dispense into sterile test tubes or petri dishes.