Medium data

Search for medium no. = [58].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

58   INORGANIC SALTS-STARCH AGAR (ISP-4)

Soluble starch  10.0     g
K2HPO4  1.0     g
MgSO4·7H2O  1.0     g
NaCl  1.0     g
(NH4)2SO4  2.0     g
CaCO3  2.0     g
Trace salts solution (see below)  1.0     ml
Agar  20.0     g
Distilled water  1.0     L
Unadjusted pH will be 7.0-7.4.
Trace salts solution:
FeSO4·7H2O  0.1     g
MnCl2·4H2O  0.1     g
ZnSO4·7H2O  0.1     g
Distilled water  100.0     ml
Comment: The premixed powder is available from Becton Dickinson & Co. as ISP Medium 4.