Medium data
Search for medium no. = [567].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
567 VENENIVIBRIO STAGNISPUMANTIS MEDIUM
| KCl | 0.5 | g |
| MgCl2·6H2O | 1.36 | g |
| MgSO4·7H2O | 7.0 | g |
| Na2S2O3·5H2O | 2.0 | g |
| CaCl2·2H2O | 0.4 | g |
| NH4Cl | 0.2 | g |
| KH2PO4 | 0.25 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| MES | 1.95 | g |
| Distilled water | 1.0 | L |
Adjust pH to 5.5 with NaOH. Distribute the medium into culture vessels (e.g., 8 ml of the medium into the Balch tubes) under a stream of a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, replace the gas phase with H2-CO2-O2 (79:15:6, v/v) gas mixture. Pressurize the inoculated culture vessels to 170 kPa with the same gas mixture.
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