Medium data
Search for medium no. = [531].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
531 BACILLUS FILIFORMIS MEDIUM
| Yeast extract (BD-Difco) | 10.0 | g |
| Sodium citrate | 3.0 | g |
| KCl | 2.0 | g |
| MgSO4·H2O | 1.0 | g |
| Na2CO3 | 3.0 | g |
| NaCl | 100.0 | g |
| MnCl2 solution (see below) | 1.0 | ml |
| FeSO4 solution (see below) | 1.0 | ml |
| Distilled water | 1.0 | L |
MnCl2 solution:
| MnCl2·4H2O | 0.36 | g |
| Distilled water | 1.0 | L |
FeSO4 solution:
| FeSO4·H2O | 50.0 | g |
| Distilled water | 1.0 | L |
Separately autoclave Na2CO3 and NaCl. Check final pH to be about 9.0.
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