Search for medium no. = [530].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
530 MODIFIED BALCH'S METHANOGENS MEDIUM
Use Medium No. 242 supplemented with 20 ml/L Fatty acid mixture (see Medium No. 266) and 1 ml/L coenzyme M solution (0.01% 2-mercaptoethanesulfonic acid, filter-sterilized).