Medium data

Search for medium no. = [514].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

514   GLUCOSE SULFIDE MEDIUM

Glucose  0.15    g
Yeast extract  1.0     g
(NH4)2SO4  0.5     g
CaCO3  0.1     g
Ca(NO3)2  0.1     g
KCl  0.05    g
K2HPO4  0.05    g
MgSO4·7H2O  0.05    g
Na2S·9H2O  0.2     g
Vitamin solution (see below)  10.0     ml
Agar  15.0     g
Distilled water  990.0     ml
Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution.
Vitamin solution:
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl·2H2O  5.0     mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
Calcium pantothenate  5.0     mg
p-Aminobenzoic acid  1.0     mg
myo-Inositol  1.0     mg
Vitamin B12  0.01    mg
Distilled water  1.0     L