Medium data
Search for medium no. = [446].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
446 MJTSO MEDIUM
| NaCl | 3.0 | g |
| MgCl2·6H2O | 0.34 | g |
| MgSO4·7H2O | 0.42 | g |
| KCl | 0.05 | g |
| K2HPO4 | 0.14 | g |
| NH4Cl | 0.25 | g |
| CaCl2·2H2O | 0.07 | g |
| Trace mineral solution (see below) | 10.0 | ml |
| Na2S2O3·5H2O | 1.0 | g |
| Yeast extract | 0.1 | g |
| Sulfur (powder) | 3.0 | g |
| NaHCO3 | 1.0 | g |
| Distilled water | 1.0 | L |
Mix ingredients except NaHCO3 and sulfur, adjust pH to 7.5 with NaOH and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.06 g sulfur) under a stream of CO2-O2 (93:7, v/v) gas mixture, seal with butyl rubber stoppers and readjust pH to 7.5 with NaOH. Pressurize the inoculated vessel to 100 kPa CO2-O2 (93:7, v/v).
Trace mineral solution:
| EDTA·2Na | 0.5 | g |
| CoCl2·6H2O | 0.15 | g |
| MnCl2·4H2O | 0.1 | g |
| FeSO4·7H2O | 0.1 | g |
| ZnCl2 | 0.1 | g |
| AlCl3·6H2O | 0.04 | g |
| Na2WO4·2H2O | 0.03 | g |
| CuCl2·2H2O | 0.02 | g |
| NiSO4·6H2O | 0.02 | g |
| H2SeO3 | 0.01 | g |
| H3BO3 | 0.01 | g |
| Na2MoO4·2H2O | 0.01 | g |
| Distilled water | 1.0 | L |
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